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KMID : 0606620070030010010
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Korean Journal of Fetal Medicine
2007 Volume.3 No. 1 p.10 ~ p.18
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Proportion of CD4+CD25+ Regulatory T Lymphocytes and Expression of Their Foxp3 in Preterm Labor
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Ma Jin-Young
Cheong Bok-Kyoung
Shim Jae-Yoon
Won Hye-Sung
Lee Pil-Ryang
Kim Ahm
Kim Yong-Man
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Abstract
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Objective: During pregnancy the immune system retains the ability to respond to foreign antigen tolerance mechanism ensure that inappropriate responses against self-antigen are prevented. Recently, the studies on the pathogenesis of preterm birth related on immunological changes of maternity and fetus has been reported, which
focused on how fetus maintains immune tolerance from the uterus. The cells that produce cytokines blocking activation or functions of T lymphocyte inhibit part of the immune responses. These inhibiting cells are called regulatory T cells (Treg). It has been reported studies on T lymphocyte inhibiting immune responses, yet in the present not many studies on the alteration of pregnancy are preformed. This study investigated that proved the immunological roles of regulatory T cells for preterm birth.
Methods: Blood samples were collected from 15 healthy women, 15 normal pregnancy and 16 preterm labor women at department of Obstetrics and Gynecology, Asan Medical Center, Seoul, Korea, from March 2006 to September 2006, were enrolled in study group. Information regarding patient and obstetrical history was recorded. The populations of
CD4+CD25high Tregs, CD4+CD25+Foxp3 Tregs and CD4+CD25highFoxp3 Tregs as a percentage of total CD4+cells were
evaluated by flow cytometric analysis. We measured the proportion of Treg cell that co-express CD25 and Foxp3 in the peripheral blood lymphocytes from preterm labor and normal pregnancy women.
Results: In preterm labor, normal pregnant women and nonpregnant healthy women, the proportion of CD4+CD25high Tregs was 0.18% (SD 0.12), 0.11% (SD 0.07), and 0.37% (SD 0.14) of the total CD4+cells respectively. The proportion of CD4+CD25high Tregs were significantly lower in preterm labor group and normal pregnant group when compared with nonpregnant women. But there was no significant difference in proportion of CD4+CD25+Tregs, CD4+CD25+Foxp3 Tregs and CD4CD25highFoxp3 Tregs comparing with nonpregnant women.
Conclusion: Our data didn’t indicate that regulatory T cells are required for the maternal immune system to tolerate the fetal allograft. But the presence of Treg cells in the peripheral blood suggests that these cells are important in
protecting the fetus from allo-active immune responses at the maternal-fetal interface. Also, further study may offer the
important key for the prevention of preterm birth and the establishment of treatment methods to increase of regulatory
T cells inducing alteration of cytokine related to preterm birth.
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KEYWORD
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CD4+CD25+ T regulatory lymphocyte, Transcription Foxp3, Preterm labor
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